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Tough Double-Network Hydrogels as Scaffolds for Tissue Engineering: Cell Behavior in vitro and in vivo Test

Tough Double-Network Hydrogels as Scaffolds for Tissue Engineering: Cell Behavior in vitro and in vivo Test
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Author(s): Jing Jing Yang (Hokkaido University, Japan), Jian Fang Liu (Hokkaido University, Japan), Takayuki Kurokawa (Hokkaido University, Japan & Creative Research Institution, Japan), Nobuto Kitamura (Hokkaido University Graduate School of Medicine, Japan), Kazunori Yasuda (Hokkaido University Graduate School of Medicine, Japan)and Jian Ping Gong (Hokkaido University, Japan)
Copyright: 2013
Pages: 10
Source title: Technological Advancements in Biomedicine for Healthcare Applications
Source Author(s)/Editor(s): Jinglong Wu (Okayama University, Japan)
DOI: 10.4018/978-1-4666-2196-1.ch023

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Abstract

Hydrogels are used as scaffolds for tissue engineering in vitro & in vivo because their three-dimensional network structure and viscoelasticity are similar to those of the macromolecular-based extracellular matrix (ECM) in living tissue. Especially, the synthetic hydrogels with controllable and reproducible properties were used as scaffolds to study the behaviors of cells in vitro and implanted test in vivo. In this review, two different structurally designed hydrogels, single-network (SN) hydrogels and double-network (DN) hydrogels, were used as scaffolds. The behavior of two cell types, anchorage-dependent cells and anchorage-independent cells, and the differentiation behaviors of embryoid bodies (EBs) were investigated on these hydrogels. Furthermore, the behavior of chondrocytes on DN hydrogels in vitro and the spontaneous cartilage regeneration induced by DN hydrogels in vivo was examined.

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