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The Detection of Tuberculosis by Loop-Mediated Isothermal Amplification (LAMP) Combined with a Lateral Flow Dipstick
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Author(s): Thongchai Kaewphinit (Srinakharinwirot University, Thailand), Somchai Santiwatanakul (Srinakharinwirot University, Thailand)and Kosum Chansiri (Srinakharinwirot University, Thailand)
Copyright: 2015
Pages: 32
Source title:
Handbook of Research on Diverse Applications of Nanotechnology in Biomedicine, Chemistry, and Engineering
Source Author(s)/Editor(s): Shivani Soni (Alabama State University, USA), Amandeep Salhotra (City of Hope National Medical Center, USA)and Mrutyunjay Suar (KIIT University, India)
DOI: 10.4018/978-1-4666-6363-3.ch013
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Abstract
Tuberculosis (TB) is an airborne infectious disease caused by the bacterium Mycobacterium Tuberculosis (MTB) and is a persistent problem in developing countries. Present methods for its detection include normal or nested Polymerase Chain Reaction (PCR) followed by electrophoresis, real-time PCR, Ziehl-Neelsen staining, and culture assay. These techniques entail various disadvantages such as high cost, long assay time and use of toxic substances. Novel loop-mediated isothermal amplification (LAMP) permits DNA to be amplified rapidly under constant temperature. The combination of LAMP and chromatographic Lateral Flow Dipstick (LAMP-LFD) by using biotinylated LAMP amplicon hybridized with Fluorescein Isothiocyanate (FITC)-labeled probes are allowed to detect MTB without electrophoresis and interpreted within 3-5 min. LAMP-LFD is as highly sensitive as PCR-electrophoresis method. Based on its sensitivity, specificity, rapidity, cost effectiveness, ease of use, and convenience, LAMP-LFD could be suitable for use in early MTB detection.
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