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HPV Detection and Genotyping Using the Luminex xMAP Technology
Abstract
The Human Papillomavirus (HPV) is playing an important role in oral cancer. The molecular detection of HPV is based on the fact that the viral DNA is present in all the epithelial layers of the affected tissue and it can be detected easily with PCR or Real-Time PCR. The major disadvantage of PCR is that it cannot provide genotype information and the Real-Time PCR can only detect very few types in a multiplex assay. Although the HPV typing assays are capable of typing a relatively large spectrum of HPV genotypes, they cannot be automated or deployed in a high-throughput platform. The bead-based technology (Luminex suspension array technology) provides a rapid and cost-effective method to simultaneously detect different HPV genotypes.
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