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Expression Characteristics and Sequence Variation Analysis of Rice Starch Regulator 1 Gene in Japonica Rice With Transgressive Variation
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Author(s): Haiying Liu (Heilongjiang Academy of Agricultural Sciences, China), Yongcai Lai (Heilongjiang Academy of Agricultural Sciences, China), Zhenhua Xu (Biotechnogogy Research Institute, Heilongjiang Academy of Agricultural Sciences, China), Zhonliang Yang (Biotechnogogy Research Institute, Heilongjiang Academy of Agricultural Sciences, China), Yanmin Yu (Biotechnogogy Research Institute, Heilongjiang Academy of Agricultural Sciences, China)and Ping Yan (Biotechnogogy Research Institute, Heilongjiang Academy of Agricultural Sciences, China)
Copyright: 2023
Volume: 14
Issue: 1
Pages: 12
Source title:
International Journal of Agricultural and Environmental Information Systems (IJAEIS)
Editor(s)-in-Chief: Frederic Andres (National Institute of Informatics, Japan), Chutiporn Anutariya (Asian Institute of Technology, Thailand), Teeradaj Racharak (Japan Advanced Institute of Science and Technology, Japan)and Watanee Jearanaiwongkul (National institute of Informatics, Japan)
DOI: 10.4018/IJAEIS.317417
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Abstract
The parents and transgressive variation lines of hybrids with significant difference in amylose content were selected to compare and analyze the accumulation characteristics of amylose and the change of OsRSR1 expression in grains in the process of grain filling, and the PCR technology was used to clone the OsRSR1 gene base sequence of four varieties. The results showed that the amylose content in grains increased gradually with grain filling process, the amylose content of offspring and parents with high amylose content were higher than the offspring and parents with low amylose content, hybrids could obtain the transgressive variation lines through the continuous directional selection of amylose content in grain, and the accumulation of amylose content in grain was closely related to genotypes. The expression quantity of OsRSR1 gene in grain was increasing during the grain filling process, the amylose content of grain was closely related to the activity of OsRSR1 gene, and the expression of grain OsRSR1 gene could also produce transgressive variation.
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