The IRMA Community
Newsletters
Research IRM
Click a keyword to search titles using our InfoSci-OnDemand powered search:
|
Using Systems Biology Approaches to Predict New Players in the Innate Immune System
Abstract
Toll-like receptors (TLRs) are critical players in the innate immune response to pathogens. However, transcriptional regulatory mechanisms in the TLR activation pathways are still relatively poorly characterized. To address this question, the author of this chapter applied a systematic approach to predict transcription factors that temporally regulate differentially expressed genes under diverse TLR stimuli. Time-course microarray data were selected from mouse bone marrow-derived macrophages stimulated by six TLR agonists. Differentially regulated genes were clustered on the basis of their dynamic behavior. The author then developed a computational method to identify positional overlapping transcription factor (TF) binding sites in each cluster, so as to predict possible TFs that may regulate these genes. A second microarray dataset, on wild-type, Myd88-/- and Trif-/- macrophages stimulated by lipopolysaccharide (LPS), was used to provide supporting evidence on this combined approach. Overall, the author was able to identify known TLR TFs, as well as to predict new TFs that may be involved in TLR signaling.
Related Content
|
S. Karthigai Selvi, Sharmistha Dey, Siva Shankar Ramasamy, Krishan Veer Singh.
© 2025.
16 pages.
|
|
S. Sheeba Rani, M. Mohammed Yassen, Srivignesh Sadhasivam, Sharath Kumar Jaganathan.
© 2025.
22 pages.
|
|
U. Vignesh, K. Gokul Ram, Abdulkareem Sh. Mahdi Al-Obaidi.
© 2025.
22 pages.
|
|
Monica Bhutani, Monica Gupta, Ayushi Jain, Nishant Rajoriya, Gitika Singh.
© 2025.
24 pages.
|
|
U. Vignesh, Arpan Singh Parihar.
© 2025.
34 pages.
|
|
Sharmistha Dey, Krishan Veer Singh.
© 2025.
20 pages.
|
|
Kalpana Devi.
© 2025.
26 pages.
|
|
|