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Genome Editing
Abstract
Targeted editing of the genomes of living organisms not only permits investigations into the understanding of the fundamental basis of biological systems but also allows to improve productively and quality of crops. This includes the creation of plants with valuable compositional properties and with traits that confer resistance to various biotic and abiotic stresses. Recently, several novel genome editing systems have been developed, which include zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALNEs), and clustered regularly interspersed short palindromic repeats/Cas9 (CRISPER/Cas9). These exciting new methods have proved themselves as effective and reliable tools for the genetic improvement of plants. The genome editing systems can also be used to exploit the genetic diversity present in the semi-domesticated and wild relatives of the cultivated crops by targeting homologous domesticated genes through allele-mining. In this chapter various tools available for gene editing, their merits, and demerits have been discussed.
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